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PhD Student - understanding of mechanisms of the regulation of photosynthesis for nitrogen fixation in Trichodesmium

PhD Student - understanding of mechanisms of the regulation of  photosynthesis for nitrogen fixation in Trichodesmium

Biology Centre of the Academy of  Science, Czech Republic


Position summary

The project of this PhD student aims at extending the understanding of mechanisms of the regulation of  photosynthesis for nitrogen fixation in Trichodesmium, for more realistic estimation of the productivity of the  oceans. This marine, filamentous, diazotrophic cyanobacterium has a particularly fast regulation of  photosynthesis. Phycourobilin isoforms turned out to be the key players in the fast regulation during the daily  activity cycle (by their reversible (un)coupling to/from photosystems) as well as in the long‐term regulation (by  changes in their expression levels) in response to iron and light stress, they shall be the main focus of this project.  This will involve UV/VIS fluorescence kinetic and FRAP measurements for investigating biophysical properties,  combined with metalloproteomics for investigating effects of iron limitation, RAMAN spectroscopy and pulsechase stable isotope labelling for analysing accumulation/transport of metabolites.  



The Department of Plant Biophysics and Biochemistry (PBB, headed by Prof. Hendrik Küpper) as part of the  Institute of Plant Molecular Biology (IPMB) within the Biology Centre of the Czech Academy of Sciences (BCAS).  The department was recently awarded a 4.9 Mio € grant from the Czech Ministry of Education with co‐financing  from the EU, which now allows for its expansion incl. additional team members and further high‐end research  instruments (e.g. ICP‐sfMS, µXRF). The department is interested in the physiology, biophysics and biochemistry  (incl. molecular biology) of photosynthetic organisms (terrestrial and submerged higher plants, algae, bacteria).  The main focus is on metal metabolism (uptake, physiological use, sequestration, complexation, detoxification,  toxicity) and photosynthesis regulation. It employs various biochemical and biophysical methods from the whole  organism to the molecular level.


More information to be found here

Biology Centre of the Academy of Science, CZ
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Printed from on 24/02/18 02:09:04 AM

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